How to Reconstitute and Inject Peptides

💧 Why do you need to reconstitute? 

You’ll notice your product comes as lyophilised (freeze dried) powder - not pre-mixed. That’s deliberate. Pre-mixed vials don’t last long in transit or storage. Powder form keeps it stable and effective for months. Once you add water, the clock starts ticking, so we let you decide when it’s time to activate it. 

💧 What is reconstitution?

To be able to use your medication, you’re going to combine it with bacteriostatic water (bac water), a process known as reconstitution. All orders come with 1 vial of 3ml of bacteriostatic water automatically included, and detailed instructions so you know what you're doing. This then creates the liquid that you will inject. 

💧 What steps are involved in reconstitution?

Reconstitution sounds fancy, but it’s really simple when you break it down. We have included detailed step by step instructions in your package (and these are further below also), but the steps are:

1. Prep and gather your supplies, sanitise hands and benchtops, remove caps off vials

2. Draw up the required bac water into your syringe

3. Add bac water into the medication vial with syringe, and gently swirl the contents to allow the powder to dissolve

4. Let it sit for a few minutes so it's fully dissolved, then your medication is ready to use straight away. 

The first time you do this it can feel a bit tricky, but you really will get the hang of it. 

✨ Reconstitution Video Guides

✨ Bonus Tips for Reconstitution

• Let the reconstituted vial sit for 5 mins before use so that any bubbles dissipate (they’re normal)

• There is no ‘correct’ amount of bac water to add to your vial - it simply changes the concentration of the medication in the water. As you add more water, the concentration of medication to water simply changes. So if you accidentally added too much water, that's no problem. You can contact us if you get stuck. 

• Some people like to reconstitute at night, and leave the product to rest in the fridge overnight so it's completely dissolved. 

• Looks like it's not quite dissolved yet? Roll it gently between your hands to agitate the product with the water. Leave it sit at room temperature for 30 minutes and it should be good to go. 

✨ Storing your Peptides after Reconstitution

• Your reconstituted vial is delicate - refrigerate immediately at 2–8 °C at the rear of your fridge. The fridge door opens and closes often and will give a variable temperature.

• Store upright, not laying on it's side

• Don’t freeze, don’t leave out at room temperature.

• Once mixed, shelf life is approx. 90 days (when mixed with bac water, sterile protocols are followed and stored in a fridge)

✨ Reconstitution Instructions

• We will send you a printed copy of the reconstitution instructions with your order. All of the instructions are also provided below, hosted in our Google Drive. 

• Important Note: instructions are different for each product and vial size

  • Each product and vial size has its own mixing instructions, so please make sure you’re following the guide for the exact product you ordered. If you're not sure, please contact us. 

  • The reason for this is concentration. Higher-strength vials contain more medication, but we usually add the same amount of bacteriostatic water so the injection volume stays small and comfortable.

  • For example, you might add 200 units of water to a 10mg vial and also 200 units to a 60mg vial. Because the second vial contains more medication but the same amount of water, it becomes more concentrated.

  • In simple terms, higher-dose vials contain more medication in the same amount of liquid, so the mixing instructions and dosing amounts will be different.

  • We have already done all these calculations for you, so that you can get it right, everytime. 

✨ Access all Reconstitution Guides in the Google Drive Links Below

You can download the PDF documents or bookmark the links so you can easily come back to them as required. 

By now, you’ve got storage and reconstitution sorted. Next up? Getting comfortable with your injections. It can feel like a big deal at first, but trust us - with a little routine, it quickly becomes second nature.

✨  Where to Inject

All peptides we supply are injected subcutaneously (just under the skin, not into muscle). The best sites are:

• Abdomen - at least 5cm away from your belly button.
  
  

• Thighs - front or outer area, avoiding veins.
  
  

• Upper arm - back of the arm, if someone else is helping.
  
  

👉 Rotate sites each week. Sticking to the same spot can cause irritation or lumps.

✨  How to Inject 

1. Reconstitute your vial of medication. Let it sit on the bench for 5 minutes before use.

2. Wash and sanitise your hands. Some people choose to wear gloves.

3. Clean the injection site with an alcohol wipe. Wait at least 1 minute for the alcohol to air dry on the skin (otherwise it can sting!)

4. Wipe the top of the medication vial with an alcohol wipe.

5. Use a syringe to draw up the correct dose. 

6. Draw air into the syringe first, and inject this air into the medication vial (helps with the pressure)

7. Pinch up a fold of skin gently between your thumb and fingers.

8. Insert the needle at a 90° angle (or 45° if you’re very lean).

9. Inject slowly and steadily - no need to rush.

10. Remove the needle, release the skin fold, and dispose of the syringe safely.

✨  What Not to Do

• Don’t inject into muscle (ouch, and not effective).
  
  

• Don’t inject into scars, stretch marks, bruises, or areas that feel hard.
  
  

• Don’t re-use syringes - single use only, always.
  
  

• Don’t skip the alcohol wipe - it’s there for a reason. If you skip swabbing the top of the vial, this increases the chance of bacteria getting in. 

✨  Routine Builder Tips

• Pair your jab with a weekly ritual (like Sunday night self-care or your favourite Netflix show).

• Keep supplies together in a clean container so it’s grab-and-go.

• Set a reminder in your phone or calendar so you never miss a dose.

• Don't forget to also set a reminder to reorder before you'll run out. 

✨  Injection Site Reactions

Injection site reactions can sometimes occur with GLP-1 medications and other peptides. These reactions are usually mild and temporary, and often become less noticeable as your body gets used to the medication over the first few weeks.

Common reactions may include:

• Mild redness at the injection site

• Slight swelling or a small bump under the skin

• Itching or mild tenderness

• Warmth around the injection area

• For GHK-Cu - a red mark that lasts a couple of days, and a stinging sensation

Tips to help reduce irritation:

• Rotate injection sites (stomach, thigh, or upper arm), and from left to right each week

• Inject into clean, dry skin and ensure you've used an alcohol wipe

• Allow refrigerated medication to reach room temperature before injecting 

• Avoid injecting into the exact same spot each time

• For GHK-Cu - add more bac water to your syringe after you draw up the dose to further dilute the peptide and reduce the stinging sensation

Most injection site reactions settle on their own within a few hours to a day and are generally harmless. If irritation persists or worsens, it’s best to pause use and seek medical advice.

✨  Bonus Video: Getting the last dose out of a vial

It's important to measure each dose for injection very carefully, and not take too much. Even just 2 units extra can add up over a month. 

If you get to the last dose in your vial, and it seems like it's not quite a full dose left, this could have happened for a few possible reasons:

1. You accidentally put in slightly less than the required units at the start. Eg. only 98 units (instead of 100) would mean you're already a little short. 

2. You accidentally took a couple more units each dose - even 2 units extra (1 additional line on syringe) will mean you've lost 6 units over 3 doses

3. The last few units can pool around the grey rubber vial stopper. Then, if you stick the syringe in quite far, you could then be above the point where the liquid is pooling.

4. (Very rare) if the vial was stored on it's side some liquid can leak, but its very rare because you've got the grey rubber stopper on the top of the vial to prevent that happening.

In actuality, it's usually a combination of 1, 2 & 3. The same number of units you put into the vial should be there to draw back out again (it can't just disappear). I would suggest that you follow some of these tips in these couple of videos which show how to get the last remaining bits out of a vial, as this should help you.

✨  Still have questions?